A humanized nanobody phage display library yields potent binders of SARS CoV-2 spike인간화된 나노바디 파지 디스플레이 라이브러리는 SARS CoV-2 스파이크의 강력한 결합제를 생성합니다Article Published on 2022-08-102022-09-11 Journal: PLoS ONE [Category] SARS, 변종, 진단, [키워드] (alpha affinity antibody antibody treatment B.1.1.7 binding modes complex effective Ex vivo humanized mitigate molecular dynamics Molecular dynamics simulation molecular dynamics simulations N501Y N501Y mutation nanobodies nanobody neutralization Neutralizing Neutralizing antibodies primary human airway RBD RBD mutation reducing SARS CoV-2 SARS-CoV-2 SARS-CoV-2 infections SARS-CoV-2 spike protein SARS-CoV-2 variants shown Spike protein Structural analysis the SARS-CoV-2 variant Viral viral burden WA1 [DOI] 10.1371/journal.pone.0272364 PMC 바로가기 [Article Type] Article
In vitro evolution predicts emerging SARS-CoV-2 mutations with high affinity for ACE2 and cross-species binding시험관 내 진화는 ACE2 및 종간 결합에 대한 높은 친화도로 새로운 SARS-CoV-2 돌연변이를 예측합니다Article Published on 2022-07-182022-09-11 Journal: PLoS Pathogens [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] ACE2 affinity being binding binding affinity binding domain boost complex Containment COVID-19 pandemic creating Cross-species transmission Cryo-electron microscopy determine determined by driven by electron microscopy Evolution high affinity human ACE2 immune evasion in vitro increase inhibit mechanism Mutation N501Y mutation predict Q498 Q498H RBD RBD variant RBD variants S477N SARS-CoV-2 SARS-CoV-2 mutation SARS-CoV-2 RBD SARS-CoV-2 receptor SARS-CoV-2 variant SARS-CoV-2 variants Transmissibility Vaccines variant variants [DOI] 10.1371/journal.ppat.1010733 PMC 바로가기 [Article Type] Article
Detection of SARS-CoV-2 RNA in wastewater, river water, and hospital wastewater of Nepal네팔의 폐수, 강물, 병원 폐수에서 SARS-CoV-2 RNA 검출Article Published on 2022-06-102022-09-11 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 applicability association biochemical marker biochemical markers collected Concentration concern coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 case CrAssphage detect detection disease Epidemiology Genome highest highlighting hospital hospitals log Mutation N501Y mutation Nepal New coronavirus peaking plant positive qPCR quantitative PCR reduction RNA concentrations SARS-CoV-2 SARS-CoV-2 RNA severe acute respiratory syndrome Coronavirus tested treated Treatment variants wastewater Wastewater treatment plant Wastewater-based epidemiology. water [DOI] 10.1016/j.scitotenv.2022.153816 PMC 바로가기 [Article Type] Article
Long-range enhancement of N501Y-endowed mouse infectivity of SARS-CoV-2 by the non-RBD mutations of Ins215KLRS and H655YIns215KLRS 및 H655Y의 비 RBD 돌연변이에 의한 SARS-CoV-2의 N501Y 부여 마우스 감염성의 장거리 향상Article Published on 2022-06-052022-09-11 Journal: Biology Direct [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] ACE2 Alpha Beta changes in cooperation Cross-species transmission disrupt ENhance facilitate flexible Gamma H655Y high infectiousness hosts Impact impacts increase Ins215KLRS insertion Interaction intramolecular interaction less Local mice mouse Mouse infection mouse-adapted strain mutant Mutation N-terminal domain N501Y N501Y mutation NTD omicron Omicron variants peptide Protein RBD Receptor binding Receptor-binding domain resulting rodents SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV-2. spike Spike protein suppressed targets the receptor-binding domain variant virus infectivity wild type [DOI] 10.1186/s13062-022-00325-x PMC 바로가기 [Article Type] Article
Increased Secondary Attack Rate among Unvaccinated Household Contacts of Coronavirus Disease 2019 Patients with Delta Variant in Japan일본에서 2019년 신종 코로나바이러스 감염증(코로나19) 환자의 예방접종을 받지 않은 가구 접촉자 중 2차 발병률 증가Article Published on 2022-03-242022-09-11 Journal: International Journal of Environmental Research an [Category] SARS, 변종, [키워드] Alpha variant Analysis attack attack rate calculated can be used Contact coronavirus disease Coronavirus disease 2019 COVID-19 Delta delta variant Delta variants enrolled evaluate household household contact household contacts household transmission Households Increased index case index cases index patient Itako Japan L452R Multivariate analysis Mutation N501Y mutation Patient Prevent Rate Regression model risk factor Risk factors SARS-CoV-2 secondary secondary attack rate spouse Transmission unvaccinated. variants wild strain [DOI] 10.3390/ijerph19073889 PMC 바로가기 [Article Type] Article
The effect of the E484K mutation of SARS-CoV-2 on the neutralizing activity of antibodies from BNT162b2 vaccinated individualsSARS-CoV-2의 E484K 돌연변이가 BNT162b2 백신을 접종한 개인의 항체 중화 활성에 미치는 영향Article Published on 2022-03-182022-09-11 Journal: Vaccine [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] antibody Antibody titer Antibody titers Beta Beta variant BNT162b2 BNT162b2 vaccine caused collected E484K E484K mutation Efficacy FIVE geometric geometric mean healthy humoral Humoral immunity Japanese Lineage N501Y mutation Neutralization antibody neutralization titer Neutralizing activity participant receiving reduced responsible SARS-CoV-2 SARS-CoV-2 variants second dose Serum Neutralization serum sample serum samples strain the SARS-CoV-2 thought vaccinated individual Vaccine vaccine efficacy variant Variant. was measured weakened were measured [DOI] 10.1016/j.vaccine.2022.02.047 PMC 바로가기 [Article Type] Article
The evaluation of potential global impact of the N501Y mutation in SARS-COV-2 positive patientsSARS-COV-2 양성 환자에서 N501Y 돌연변이의 잠재적 글로벌 영향 평가Article Published on 2022-03-012022-09-11 Journal: Journal of medical virology [Category] SARS, 변종, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Affect age Analysis approach average Chain Reaction Contagion contribute coronavirus evaluate examined FAM female global pandemic groups Health authority male medium Mutation mutation rate mutations N501Y N501Y mutation Patient PCR PCR test polymerase chain polymerase chain reaction Polymerase chain reaction (PCR) positive positive patient positive sample Precaution Public public health public health authorities quantitation Randomly ranged Rapid retrospective reverse transcription PCR reverse transcription PCR (RT-PCR). RT-PCR SARS-CoV-2 severe acute respiratory syndrome Coronavirus Spread virus [DOI] 10.1002/jmv.27413 PMC 바로가기 [Article Type] Article
Real-Time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with B.1.1.7 Variant of ConcernB.1.1.7 우려 변이체와 관련된 SARS-CoV-2의 스파이크 단백질에서 N501Y 돌연변이 검출을 위한 실시간 RT-PCR 대립형질 판별 분석Research Article Published on 2022-02-162022-09-10 Journal: Microbiology Spectrum [Category] COVID19(2023년), MERS, SARS, 변종, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 allelic Amino acid B.1.1.7 B.1.351 B.1.617.2 caused characterized circulating concern coronavirus coronavirus disease Coronavirus disease 2019 costly COVID-19 Critical current decrease demonstrated described detection discrimination displaying effort example focus help identify Intensive labor Lineage mutant Mutation N501Y N501Y mutation negative nucleotide P.1 pandemic PCR assay polymorphism positive public health real-time reverse transcriptase real-time RT-PCR respiratory reverse transcriptase reverse transcription PCR RT-PCR RT-PCR assay Sample Sanger sequencing SARS-CoV-2 SARS-CoV-2 lineage sequenced Sequencing several variant several variants severe acute respiratory syndrome Coronavirus single nucleotide Single nucleotide polymorphism SNP specimen spike gene Spread Surveillance tested the Spike turnaround times variants of concern virus VoC VOCs whole-genome whole-genome sequencing wild type [DOI] 10.1128/spectrum.00681-21 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 wastewater surveillance in Germany: Long-term RT-digital droplet PCR monitoring, suitability of primer/probe combinations and biomarker stability독일의 SARS-CoV-2 폐수 감시: 장기 RT-디지털 액적 PCR 모니터링, 프라이머/프로브 조합의 적합성 및 바이오마커 안정성Case Reports Published on 2022-02-152022-09-11 Journal: Water research [Category] COVID19(2023년), MERS, SARS, 바이오마커, 변종, 치료기술, 치료제, [키워드] approach assays B.1.1.7 Biomarker Biomarker stability Case report circulation city Clinical data Clinical tests Combination Concentration conditions contributing to copy numbers coronavirus coronavirus. correlation COVID-19 case detect determine Digital Digital droplet PCR dissolved oxygen Early detection early stage Epidemiology experiment Germany glycol management methodology N501Y N501Y mutation Nsp3 Nsp9 Occurrence ORF ORF1 ORF1ab oxygen pandemic PCR PCR analysis Polyethylene glycol primer probe proportion reported robust SARS-CoV-2 SARS-CoV-2 RNA Seven shown Spread stability Surveillance target target gene Target genes the SARS-CoV-2 titre Treatment variant variants variants of concern Viral Viral RNA was used wastewater Wastewater-based epidemiology wildtype [DOI] 10.1016/j.watres.2021.117977 PMC 바로가기 [Article Type] Case Reports
Molecular and Epidemiological Characterization of Emerging Immune-Escape Variants of SARS-CoV-2Medicine Published on 2022-02-102022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] acute respiratory syndrome Analysis antibodies Beta BNT162b2 mRNA vaccine booster vaccination characterization circulating circulating variants conducted coronavirus coronavirus disease COVID-19 COVID-19 vaccine Delta displaying dual antibody cocktail therapy E484K Efficacy emerging variants epidemiological survey exhibited Gamma identify individual management monoclonal antibody mRNA vaccine N501Y mutation neutralization Neutralizing Neutralizing activity Neutralizing antibodies pandemic prevalent SARS-CoV-2 SARS-CoV-2 variant shown significantly Spike protein therapeutic vaccination Vaccine vaccinee variant variants Virus-like particle [DOI] 10.3389/fmed.2022.811004 PMC 바로가기 [Article Type] Medicine